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	Provedor de dados ArchiMer (1) Electron. J. Biotechnol. (1) Autor Busse,Nadine (1) Chollet, Bruno (1) Czermak,Peter (1) Dubreuil, Christine (1) Dégremont, Lionel (1) Garcia, Celine (1) Godfrin, Yoann (1) Gonçalves De Sa, Amélie (1) Mesnil, Aurelie (1) Moussa Pouly, Mirna (1) Oppermann,Tobias (1) Schwarz,Stefan (1) Serpin, Delphine (1) Tourbiez, Delphine (1) Travers, Marie-agnes (1) Mais... Palavra-chave Bacterial pathogen (2) ATP assay (1) Bivalvia (1) Bovine respiratory disease (1) Cattle industry (1) Cockle (1) Critical parameters (1) Lipopolysaccharides (1) Mannheimia haemolytica (1) Microbial biotechnology (1) Mortality (1) Polymyxin B (1) Quantification (1) Shellfish (1) Vibrio aestuarianus (1) Virulence (1) Mais... Tipo do documento Journal article (1) Text (1) Ano 2021 (1) 2016 (1) País Chile (1) France (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A fast and simple assay to quantify bacterial leukotoxin activity Electron. J. Biotechnol. Oppermann,Tobias; Schwarz,Stefan; Busse,Nadine; Czermak,Peter. Background: Mannheimia haemolytica is the primary bacterial pathogen in causing bovine respiratory disease with tremendous annual losses in the cattle industry. The leukotoxin from M. haemolytica is the predominant virulence factor. Several leukotoxin activity assays are available but not standardized regarding sample preparation and cell line. Furthermore, these assays suffer from a high standard error, a prolonged time consumption and often complex sample pretreatments, which is important from the bioprocess engineering point of view. Results: Within this study, an activity assay based on the continuous cell line BL3.1 combined with a commercial available adenosine triphosphate viability assay kit was established. The leukotoxin activity was found to be... Tipo: Journal article Palavras-chave: ATP assay; Bacterial pathogen; Bovine respiratory disease; Cattle industry; Critical parameters; Lipopolysaccharides; Mannheimia haemolytica; Microbial biotechnology; Polymyxin B; Quantification; Virulence. Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600006 Vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles Cerastoderma edule in France, and establishment of Vibrio aestuarianus subsp. aestuarianus subsp. nov. and Vibrio aestuarianus subsp. francensis subsp. nov. ArchiMer Garcia, Celine; Mesnil, Aurelie; Tourbiez, Delphine; Moussa Pouly, Mirna; Dubreuil, Christine; Gonçalves De Sa, Amélie; Chollet, Bruno; Godfrin, Yoann; Dégremont, Lionel; Serpin, Delphine; Travers, Marie-agnes. Cockle mortality events have been reported in northern France since 2012. In the present study, we describe and investigate the implication of a potential bacterial causative agent in cockle mortality. Bacteria isolated from five different cockle mortality events were characterized and studied. Using phenotypic analysis combined with DNA–DNA hybridization (DDH) and whole genome sequencing, the isolates were shown to belong to Vibrio aestuarianus , a species regularly detected in France during oyster mortality events. Comparison of the strains from cockles with strains from French oysters and the type strain showed that the strains from cockles were genetically different to those from oysters and also different to the V. aestuarianus type strain. Moreover,... Tipo: Text Palavras-chave: Bacterial pathogen; Bivalvia; Cockle; Mortality; Shellfish; Vibrio aestuarianus. Ano: 2021 URL: https://archimer.ifremer.fr/doc/00677/78893/81195.pdf Registros recuperados: 2 Primeira ... 1 ... Última

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